January 2022

Aim: The present study was undertaken to evaluate the concentration of Periodontal Ligament Associated Protein-1 (PLAP-1) and Tumour Necrosis Factor – Alpha (TNF-α) in Gingival Crevicular Fluid (GCF) and serum in healthy, periodontitis (P) and P participants after the Non-Surgical Periodontal Therapy(NSPT). Materials and Methods: 26 participants with age (25-50yrs) was included in study. Based on clinical and radiological parameters these participants were divided as; group 1: healthy, group 2: P, group 3: P after treatment. PLAP-1 and TNF-α levels in GCF and serum, periodontal parameters like Plaque Index (PI), Gingival Index (GI), Probing Pocket Depth (PPD),Clinical Attachment Level (CAL) were measured at baseline and 4-6 weeks after NSPT. Results: The results of the study indicated that the mean PLAP-1 increased in P participants (2842.56±1004.72pg/ml & 2292.63±495.01) and TNF-α (15.80±0.71pg/ml &13.98±0.3pg/ml) levels in both GCF and serum .In treated P group there was statistically significant reduction in PLAP-1 (927.38±266.59pg/ml and 975.94±221.88pg/ml) and TNF-α (3.73±0.20pg/ml and 4.09±0.59pg/ml) concentration in both GCF and serum respectively. Conclusion: There is substantial increase in the concentration of PLAP-1 & TNF-α in GCF and serum in periodontitis. Treatment of periodontal disease leads to a reduction in levels of PLAP-1 & TNF-α.