Grape Seed Extract Down-modulates Oxidative and Inflammatory Responses of Oral Mucosa Cells

Authors: Villas Boas SB, Sousa LS, Cardoso LM, Tavella-Silva NC, de Souza Costa CA and Basso FG


Background: Proanthocyanidins (PAs) are bioflavonoids extracted from seeds and fruits that act as cross-linkers in collagen molecules and act as matrix metalloproteinase (MMP) regulators. Therefore, the use of these compounds seems to be a potential adjunctive therapy for traditional periodontal treatment. This study investigated the potential of PAs extracted from grape seeds (grape seed proanthocyanidins extract – GSPE) to modulate the oxidative and inflammatory response of oral mucosa cells. Materials and methods: Non-cytotoxic concentrations of GSPE were selected for oral keratinocytes and gingival fibroblasts. Cells were seeded in 96-well plates and exposed to tumor necrosis factor-alpha (TNF-α) (100 ng/mL) and then treated with GSPE (0.001%) in serum-free culture medium. After 24-h-incubation, oxidative cells response (synthesis of nitric oxide), synthesis of interleukin-6, MMP-2, MMP-3, and MMP-9 was measured by ELISA immunoassay, as well as gelatinolytic activity (zymography). Data were analyzed by analysis of variance and Tukey tests (a = 0.05). Results: TNF-α-challenged cells exhibited the highest rates of NO and inflammatory mediators’ synthesis. Reduction of NO synthesis, downregulation of interleukin-6, MMP- 2, and -9, as well as decrease in the gelatinolytic activity of fibroblasts was observed in GSPE groups. Conclusions: GSPE is capable of modulating the oxidative and inflammatory responses of oral mucosa cells.

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